Sequences of NASBA oligonucleotide primer, RT-PCR oligonucleotide primer and BIO-labelled oligonucleotide probe.
| Oligo sequence (5′->3′) | Amplicon (nt) | GenBank accession no | |
| Nucleic acid sequence-based amplification primer | |||
| RPS18_P1 (T7) | aattctaatacgactcactatagggagaaggCAGACAAGGCCTACAGACTT | 149 | NM_022551.2 |
| RPS18_P2 | AACTCACTGAGGATGAGGTG | ||
| HER2_P1(T7) | aattctaatacgactcactatagggagaaggGAGCCAGCCCGAAGTCTGTA | 144 | NM_000125.2 |
| HER2_P2 | AGGACCTGCTGAACTGGTGTA | ||
| ERα_P1(T7) | aattctaatacgactcactatagggagaaggCTCCACCATGCCCTCTACACA | 148 | NM_000125.2 |
| ERα_P2 | TGATGATTGGTCTCGTCTGG | ||
| MMP11_P1(T7) | aattctaatacgactcactatagggagaaggCAGTGGGTAGCGAAAGGTGT | 141 | NM_005940 |
| MMP11_P2 | AGACCTGGACTATCGGGGAT | ||
| YBX1_P1 (T7) | aattctaatacgactcactatagggagaaggGGTAAGCCGGCATTTACTCA | 151 | NM_004559.3 |
| YBX1_P2 | GGTACCGCCGCAACTTCAAT | ||
| CASP8_P1 (T7) | aattctaatacgactcactatagggagaaggCAGATGAAGCAGTCCATGTTA | 140 | NM_001228.4 |
| CASP8_P2 | GATGCAGGGGCTTTGACCAC | ||
| SOD2_P1(T7) | aattctaatacgactcactatagggagaaggCCGTAGTCGTAGGGCAGGT | 155 | NM_000636.2 |
| SOD2_P2 | CGGCTTCAGCAGATCGGCGG | ||
| Probe | |||
| RPS18 | BIO-CGTGGGTGTGTCCAAGAAGAAAT | ||
| HER2 | BIO-AAGAGTCCCAACCATGTCAAAA | ||
| ESR1 | BIO-TACTGTTTGCTCCTAACTTGCTCTTGGACAG | ||
| MMP11 | BIO-TGAGACCTGGACTATCGGGGATGA | ||
| YBX1 | BIO-GCGGAGGCAGCAAATGTTACAGGTC | ||
| CASP8 | BIO-TCATTTTGAGATCAAGCCCCACGAT | ||
| SOD2 | BIO-TTTGGGGTATCTGGGCTCCA | ||
| RT-PCR primer | |||
| RPS18_P1 | TACTCAACACCAACATCGATGGGC | 100 | |
| RPS18_P2 | GCTTTCCTCAACACCACATGAGCA | ||
| HER2_P1 | TAGACCATGTCCGGGAAAAC | 99 | |
| HER2_P2 | GCACATCCTCCAGGTAGCTC | ||
| ERα_P1 | ATGATCAACTGGGCGAAGAG | 92 | |
| ERα_P2 | AGGATCTCTAGCCAGGCACA | ||
| MMP11_P1 | AGGGCCACATTTGGTTCTTC | 117 | |
| MMP11_P2 | CAGCATGGACCGGGAACCT | ||
| YBX1_P1 | TGGTTCAATGTAAGGAACGGA | 114 | |
| YBX1_P2 | ACTGCGAAGGTACTTCCTGG | ||
| CASP8_P1 | CAGGAATGGAACACACTTGGA | 159 | |
| CASP8_P2 | CAGATGAAGCAGTCCATGTTA | ||
| SOD2_P1 | CACCAGCACTAGCAGCATGT | 123 | |
| SOD2_P2 | CCGTAGTCGTAGGGCAGGT | ||
BIO, biotin; CASP8, caspase 8; ERα, estrogen receptor alpha; HER2; epidermal growth factor receptor 2, MMP11, matrix metallopeptidase 11; RPS18, ribosomal protein S18; SOD2, superoxide dismutase 2; YBX1, Y box binding protein.
NASBA and RT-PCR oligonucleotide primer and probes are indicated in upper-case characters. The 3′ antisense NASBA oligonucleotide primers are elongated with a T7-promotor recognition sequence indicated in lower-case italic characters.