Table 1

Mutation detection. Quick-multiplex-consensus-PCR and high-resolution melting analysis

Sequencing analysis of hotspots
MutantWild-type
In cell linesKRAS & BRAF onlyMutant230(PPV 100%)
Wild-type0122(NPV 100%)
All hotspotsMutant400(PPV 100%)
Wild-type(250—not validated by sequencing)
In FFPE tumour samplesKRAS & PIK3CA onlyMutant165(PPV 76%)
Wild-type065(NPV 100%)
All hotspotsMutant367(PPV 84%)
Wild-type(387—not validated by sequencing)
  • FFPE, formalin-fixed paraffin-embedded; NPV, negative predictive value; PPV, positive predictive value.