The combined use of complete IGH and IGK rearrangement assays improves clonality detection in formalin-fixed, paraffin-embedded follicular lymphoma samples
| Combined BIOMED-2 PCR assays | No. of assays | Clonality detection rate (%), n=118 | Samples with a single clonal result (%), n=118 | |
| IGH | Vн-FR1-Jн, Vн-FR2-Jн, Vн-FR3-Jн | 3 | 79.7 | 19.5 |
| IGK | Vκ-Jκ, Vκ/intron-Kde | 2 | 82.2 | 48.3 |
| IGH + IGK | Vн-FR2-Jн, Vκ-Jκ | 2 | 86.4 | 39.0 |
| IGH + IGK | Vн-FR2-Jн, Vн-FR3-Jн, Vκ-Jκ | 3 | 91.5 | 31.4 |
| IGH + IGK | Vн-FR2-Jн, Vн-FR3-Jн, Vκ-Jκ, Vκ/intron-Kde | 4 | 94.9 | 20.3 |
| IGH + IGK | Vн-FR1-Jн, Vн-FR2-Jн, Vн-FR3-Jн, Vκ-Jκ, Vκ/intron-Kde | 5 | 94.9 | 15.3 |
Stepwise combinations of complete IGH and IGK rearrangement assays are shown in rows 3–6. Only data for the best combination per number of assays (2, 3, 4 and 5) are shown.