Primer sequences used for this study
| Gene | Sequence (5′–3′) |
| Cyr61 | F: CCTTGTGGACAGCCAGTGTA |
| R: TGTAGAAGGGAAACGCTGCT | |
| CTGF | F: CCTGGTCCAGACCACAGAGT |
| R: GTAATGGCAGGCACAGGTCT | |
| Glyceraldehyde-3-phosphate dehydrogenase | F: CAAGAGCACAAGAGGAAGA |
| R: ACTGTGAGGAGGGGAGATTC | |
| 18s F | GCT GAG AAG ACG GTC GAA CT |
| CTGF F | CCA AAA GTT ACA TGT TTG CAC CT |
| Cyr61 F | GGA GGG AGG GGA AAT GTA AT |
| Universal reverse primer | CAC AAC GAG ACG ACG ACA AC |
Cysteine rich-61 (Cyr61), connective tissue growth factor (CTGF) and glyceraldehyde-3-phosphate dehydrogenase primer sets were used in the in vitro analysis of hypoxic induction of the CCN genes.
Primer sequences 18s F, CTGF F and Cyr61 F used for quantitative RT-PCR on paraffin-embedded tissues. The universal reverse transcriptase primer includes the ambiguity codes VN, where V is A, C or G, and N can be any nucleotide A, C, G or T. The universal reverse primer is a common reverse primer for all subsequent PCR reactions.