Gene | Forward primer (5′–3′) | Reverse primer (5′–3′) | Reference |
XAF1 | Zou et al9 | ||
M-MSP | TTTGTAAGAAACGAAATTTAATCGA | CCTACCCTTAAAACCCACGAT | |
U-MSP | TTTGTAAGAAATGAAATTTAATTGA | CTCCTACCCTTAAAACCCACAAT | |
CDH13 | Roman-Gomez et al10 | ||
M-MSP | TCGCGGGGTTCGTTTTTCGC | GACGTTTTCATTCATACACGCG | |
U-MSP | TTGTGGGGTTGTTTTTTGT | AACTTTTCATTCATACACACA | |
JUNB | Yang et al11 | ||
M-MSP | GACGTTAGGAAAGTTATCGC | CGAACTAAATACCTAATCGCG | |
U-MSP | TTGGGGGAAATGATGTTAGGAAAGTTATTGT | ACTACAACAAACAACAAACTCTCCACTACA |
Primer sequences kindly offered by S Baylin.7
M-MSP, MSP chain reaction for the methylated allele; Tm, annealing temperature; U-MSP, MSP chain reaction for the unmethylated allele.