mRNA expression for the mesenchymal stem cell-specific markers, CD29, CD44 and CD90, was analysed from four proliferating infantile haemangioma (IH) cell isolates (P1–P4). RNA was extracted from 1×10⁵ cells, reverse-transcribed into cDNA, and analysed by quantitative real-time (qRT-PCR. Expression of transcripts was determined relative to glyceraldehyde-3-phosphate dehydrogenase (∆Ct). The table gives the ∆Ct values obtained for each gene. The passage number is given in parentheses; ‘–’ shows no amplification and therefore the absence of mRNA transcript. The mean ∆Ct value (±SEM) across the four IH cell isolates (P3) for CD29, CD44 and CD90 was 1.75 (±0.4), 3.4 (±0.4) and 3.2 (±0.7), respectively.