Change in peroxisome proliferator-activated receptor (PPAR-γ) and osteonectin mRNA expression in haemangioma explant-derived cells (HaemEDCs) before and after differentiation
| ∆∆Ct PPAR-γ | ∆∆Ct osteonectin | |
| Haem-P1 | 4.7 | 3.4 |
| Haem-P2 | 4.3 | −1.5 |
| Haem-P3 | 5.5 | 1.7 |
| Haem-P4 | 4.9 | −0.2 |
| Haem-P5 | 2.9 | −1.0 |
| Mean difference in gene expression | 4.7 | 0.5 |
| SEM | 0.4 | 0.9 |
Cell isolates from explant culture of five proliferating infantile haemangiomas (His), designated HaemEDCs-1, HaemEDCs-2, HaemEDCs-3, HaemEDCs-4 and HaemEDCs-5, were differentiated into adipocytes and osteoblasts. Quantitative real-time (qRT)-PCR was performed to identify changes in PPAR-γ and osteonectin mRNA expression in undifferentiated cells versus differentiated cells (∆∆Ct).