Scores for tissue microarray (TMA) samples (1–13) are shown for each laboratory (A–M) participating in mutation analysis of EGFR and/or KRAS in 2009. Boxes: light grey, consensus score; black, wrong answer (false positive or false negative); grey, the test was received by the laboratory, but no test result was provided. %, wrong answer with low percentage of tumour cells, implying that in a clinical setting the test would not be performed because the tumour cell fraction was too low.
TMA samples: 1, KRAS mutation c.34G>T (p.Gly12Cys or p.G12C); 2, EGFR mutation c.2235_2249del (p.Glu746_Ala750del or p.E746_A750del); 3 and 5, KRAS mutation c.35G>A (p.Gly12Asp or p.G12D); 8, EGFR mutation c.2303_2311dup (p.Ser768_Asp770dup or p.S768_D770dup); 9, EGFR mutation c.2235_2249del (p.Glu746_Ala750del or p.E746_A750del); 12, EGFR mutation c.2240_2254del (p.Leu747_Thr751del or p.L747_T752del); 13, EGFR mutation c.2237_2253delinsTTGCT (p.Glu746_Thr751delinsValAla or p.E746_T751delinsVA). Samples 3 and 5 are duplicates. 4,6,7,10 and 11 are wild type = no mutation for EGFR or KRAS.