Table 4

Analysis of PTEN alterations in gastric adenocarcinoma using different approaches

Author	N	Events (%)	Methods	Findings in detail	Association with
Author	N	Events (%)	Methods	Findings in detail	pT	pN	UICC stage	Grading WHO	Lauren
Wen et al 20	144	18.6	SSCP-PCR; DNA- sequencing	15 missense mutations in exons 1, 2, 4 and 9; 9 nonsense mutations in exons 1, 7 and 9 2 1-bp deletions in exon 4	NI	NI	<0.005	<0.05	NI
Guo et al 21	53	9.4	SSCP-PCR; DNA- sequencing	1 stop mutation in exon 7; 2 point mutations in exons 5 and 7; 1 single base A mutation in exon 5	<0.05^*	<0.05^*	<0.005^*	NI	NI
Lima et al 22	48	2.1	SSCP-PCR; DNA- sequencing	1 silent point mutation in exon 8	NI	NI	NI	NI	NI
Li et al 15	60	5	SSCP-PCR; DNA- sequencing	One base change in exons 5 and in exon 8 One 4-bp deletion in exon 8 All only in advanced cases	NI	NI	NI	NI	NI
Wang et al 23	60	28.3	SSCP-PCR; DNA- sequencing	8 missense mutations in exons 3, 4, 5 and, 6; 5 silent mutations in exons 2 and 4; 2 nonsense mutations in exon 3; 1 12-bp deletion in exon 4	NI	NI	NI	NI	NI
Sato et al 24	58	1.7	SSCP-PCR; methylation-specific PCR; DNA- sequencing	1 5-bp deletion in intron 7; No mutation in coding regions; No promoter methylation	NI	NI	NI	NI	NI
Chang et al 17	15	0	SSCP-PCR; DNA- sequencing	No point mutations or base changes; In 33% (5/15) smaller size transcripts	NI	NI	NI	NI	NI
Kang et al 25	66	39.4	Methylation-specific PCR;	73% (19/26) in examined cases with negative PTEN expression (IHC staining)	<0.01^*	<0.001^*	<0.001^*	NS^*	NS^*