Antigen | Manufacturer | Antibody clone | Dilution | Antigen retrieval | Incubation |
MMP-1 | Abcam | Rb pAb | 1:3000 | Citrate (pH 6) | Overnight |
MMP-2 | Abcam | Rb pAb | 1:50 | Citrate (pH 6) | 30 min |
MMP-7 | Abcam | Rb pAb | 1:50 | Citrate (pH 6) | Overnight |
MMP-9 | Dako | Rb pAb | 1:30 | Citrate (pH 6) | 1 h |
TGF-ß1 | Abcam | Mouse mAb | 1:400 | Citrate (pH 6) | 1 h |
VEGF | Abcam | 5C3-F8 | 1:100 | Citrate (pH 6) | 1 h |
E-cadherin | Invitrogen | Mouse Ab | 1:100 | Citrate (pH 6) | 1 h |
MMP, matrix metalloproteinase; TGF, transforming growth factor receptor; VEGF, vascular endothelial growth factor.