Table 1

Details of antibodies used in the current study

AntigenManufacturerAntibody cloneDilutionAntigen retrievalIncubation
MMP-1AbcamRb pAb1:3000Citrate (pH 6)Overnight
MMP-2AbcamRb pAb1:50Citrate (pH 6)30 min
MMP-7AbcamRb pAb1:50Citrate (pH 6)Overnight
MMP-9DakoRb pAb1:30Citrate (pH 6)1 h
TGF-ß1AbcamMouse mAb1:400Citrate (pH 6)1 h
VEGFAbcam5C3-F81:100Citrate (pH 6)1 h
E-cadherinInvitrogenMouse Ab1:100Citrate (pH 6)1 h
  • MMP, matrix metalloproteinase; TGF, transforming growth factor receptor; VEGF, vascular endothelial growth factor.