EQA run | EQA case | Validated BRAF genotype | Estimated neoplastic cell content (%) |
---|---|---|---|
1 | 1 | No mutations detected at BRAF codon 600 | 65–80 |
2 | c.1799T>A p.(Val600Glu) | 80–85 | |
3 | c.1799T>A p.(Val600Glu) | 80–95 | |
2 | 4 | c.1798_1799delinsAA p.(Val600Lys) | 30–50 |
5 | No mutations detected at BRAF codon 600 | 80 | |
6 | No mutations detected at BRAF codon 600 | 30–40 | |
3 | 7 | c.1799T>A p.(Val600Glu) | 35 |
8 | No mutations detected at BRAF codon 600 | 70–80 | |
9 | c.1799T>A p.(Val600Glu) | 60–70 |
Mutation nomenclature follows HGVS guidelines using GenBank accession number NM_004333.4.
EQA, external quality assessment; HGVS, Human Genome Variation Society.