Primers and probes used for screening
| JAK2 (V617F) | Exon12_JAK2 | MPL515 |
|---|---|---|
| Forward primer GCAGCAAGTATGATGAGCAAGCT | Forward primer CATACTTTCAGTGTATTTTGAAGTG | Forward primer TGGTGACCGCTCTGCATCTA |
| Reverse primer GGCATTAGAAAGCCTGTAGTTTTACTTAC | Reverse primer ATGTCACATGAATGTAAATCAAG | Reverse primer TCCACCGCCAGTCTCCTG |
| Probe WT_FAM_MGB TGGAGTATGTGTCTGTGGA | – | Probe WT_VIC_MGB TGAGGTGGCAGTTTC |
| Probe V617F_VIC_MGB TGGAGTATGTTTCTGTGGAG | – | Probe W515L_FAM_MGB CTGCTGAGGTTGCAGTT |
| – | – | Probe W515K_FAM_MGB CTGCTGAGGAAGCAGT |