Case 4 genotyping errors and methods used
| Error | Methods | Number of laboratories |
|---|---|---|
| No mutation detected at codon 600 | Pyrosequencing | 2 |
| Roche cobas | 1 | |
| p.(Val600Glu) reported | Pyrosequencing | 3 |
| Real time PCR | 2 | |
| Sanger sequencing | 1 | |
| Mass spectrometry | 1 | |
| High resolution melt analysis and Sanger sequencing | 1 | |
| High resolution melt analysis and SNaPShot analysis | 1 | |
| Real time PCR, Sanger sequencing and Entrogen kit | 1 | |
| Incomplete test performed—see text | Qiagen RGQ PCR kit | 1 |