Table 1

Number of harvested lymph nodes

Lymph node revealing solutionManual dissectionFat clearanceStatistically significant difference?
No. casesMean no. lymph nodesNo. casesMean no. lymph nodes/(+) additional nodes
Acetone*25 34
 80
15.4
6.9
34
80
+5.7
+4.4
Acetone/IPA/oil2686427.0
Acetone/xylene22 752.775+7.5
Acetone/alcohol/xylene18 224.71030.9Yes (p<0.0001)
Acetone/alcohol/xylene27 1520.915+68.6
Acetone/alcohol/xylene2831174.3
Alcohol23 4819.448+23.6No (p=0.177)
Alcohol†29 829.6
5.2
15527.6
20.4
Yes (p<0.001)
Yes (p<0.001)
Alcohol332734
Alcohol‡19 3718.1/21.2140/18276.4/73.7
Alcohol/xylene31 412143−9/−4
Alcohol/xylene30 1018.710−2.3No (p=0.57)
Alcohol/xylene321036.2103+12.4
Alcohol/xylene§21221
 50
10.5
13.1
5123.1Yes (p<0.001)
Alcohol/xylene17 417.341+47.6
Alcohol/xylene204850.2
GEWF37 4018.304519.96No (p=0.53)
GEWF¶42 30
 12
5.1
6.25+1.6
30
12
+1.73
+1.2
Yes (p<0.01)
Yes (p<0.01)
GEWF36 326.83510.2Yes (p=0.002)
GEWF41 302.9430+8.6
GEWF40 356.2635+13.0
GEWF‡38 5910
9
6117
16
Yes (p<0.001)
GEWF391175.012513.0Yes (p<0.001)
GEWF35 345.95914.7Yes (p=0.05)
GEWF342311.4423+6.0
GEWF**43 7662
GEWF44  87.68+4.7Yes (p<0.5)
  • *Two study groups.

  • †Non-neoadjuvant/neoadjuvant.

  • ‡Colonic/rectal.

  • §Multiple sites with lymph node clearance performed at the main site only.

  • ¶Two study groups, multiple dissections and multiple sites of tumour.

  • **Comparison of cases from different years; also includes assessment of improved surgical practice.

  • GEWF, glacial acetic acid, ethanol, water and formalin.