Grading system for immunohistochemical staining
| Grade | IL-6 liver | CRP liver | CRP heart | P-selectin | ICAM-1 |
|---|---|---|---|---|---|
| 0 | Negative | Negative or <1 hepatocyte per 50 staining positive with no specific pattern | Negative | Negative | Negative |
| 1 | Focal staining | Periportal staining with intervening negative staining | Focal staining | Positive | <10% vessels/sinusoids staining positive |
| 2 | Diffuse staining | Strong periportal staining with positive intervening hepatocytes | Strong zonal staining with positive intervening myocytes | – | Between 10% and 50% of vessels/sinusoids stain positive |
| 3 | – | Strong diffuse staining | Strong diffuse staining | – | >50% of vessels/sinusoids stain positive |
If not otherwise specified, grading was used for heart and liver tissues. P-selectin staining patterns were either completely negative or diffusely strongly positive. VCAM-1 staining was diffusely positive for all endothelium within the heart and liver.
CRP, c-reactive protein.