Antibodies used for immunohistochemistry
| Antibodies | Clone | Manufacturer | Localisation | Positive control |
|---|---|---|---|---|
| SHH | H-160 | Santa Cruz, Santacruz, California, USA | Cytoplasm | Endothelia |
| GLI1 | H-300 | Santa Cruz, Santacruz, California, USA | Nuclei and cytoplasm | Glioma cells |
| GLI2 | Polyclonal | Abcam, Cambridge, Massachusetts, USA | Nuclei and cytoplasm | Glioma cells |
| GLI3 | H-280 | Santa Cruz, Santacruz, California, USA | Nuclear membrane | Endothelia |
| ABCG2 | BXP-21 | Abcam, Cambridge, Massachusetts, USA | Cytoplasm | Endothelia |
ABCG2, ABC binding cassette G2; GLI, glioma-associated oncogene homologue; SHH, Sonic hedgehog.