Table 2

Overview of mutation detection performance of each laboratory

Lab ALab BLab CLab DLab ELab FLab GLab HLab ILab JLab KLab LLab M
EGFR
Detection Method		SangerPyrosequencingqPCRNTNTqPCRqPCRSangerqPCRNGSNTSangerSanger
BRAF
Detection Method		qPCRPyrosequencingqPCRSangerNTqPCRqPCRSangerPyrosequencingNGSPyrosequencingPyrosequencingSanger
Specimen 7
 EGFR exon 18
(33% G719S)		NoYesNTNTNTYesNTYesYesYesNTYesYes
 BRAF exon 15
(25% V600E)		NoYesYesYesNTYesYesYesYesYesNoYesYes
Specimen 8
 EGFR exon 21
(20% L858R)		YesYesYesNTNTYesYesNoYesYesNTYesYes
 BRAF exon 15
(66% V600E)		YesYesYesYesNTYesYesYesYesYesNoYesYes
Total score2/44/43/32/2NT4/43/33/44/44/40/24/44/4

  • Total number of potential calls=42. Total number of missed calls=5. Failure rate 5/42=11.9%. Laboratories were not assessed for mutations that they did not routinely provide clinical screening.

  • NGS, next-generation sequencing; NT, not tested, denotes those mutations that laboratories did not routinely screen; EGFR, epidermal growth factor receptor.