Table 2

Technical failure rates for reverse transcription-PCR (RT-PCR) in formalin-fixed, paraffin-embedded material from soft-tissue tumour specimens for the three time periods assessed

Year/type of sections receivedTotal no. of requestsNo. and % of RT-PCR technical failsNo. of fails from in-house materialNo. of fails from referred materialPercentage of referral case which failedNo. of failed cases from biopsy materialNo. of failed cases from excision materialNo. of RT-PCR technical fails that also had FISH, and no. with successful FISH
2011 (5×10 µm scrolls)20928 (13.4%)8 (6 excision specimens, 2 core biopsies)20 (9 excision specimens, 1 biopsy, 10 unknown)20/28=71.4%315 (NB in 10 cases material type was not known)17/17
2012 (5×5 µm slides)1587 (4.4%)1 (1 core biopsy)6 (5 excision specimens, 1 core biopsy)6/7=85.7%257/7
2013 (1×10 µm slide)20316 (7.9%)11 (11 biopsies)5 (3 excision specimens, 2 biopsies)5/16=31.3%13314/14
  • FISH, fluorescence in situ hybridisation.