Table 1

Idylla EGFR Mutation Assay performance on archival extracted DNA

Case	% Neoplastic cells	DNA (ng/µL)	Reference method	Idylla
1	80	60	WT	WT
2	60	60	L858R	L858R
3	40	60	WT	WT
4	50	7.15	DEL19	DEL15
5	20	6.9	DEL19	DEL15
6	70	60	WT	WT
7	30	4.94	DEL19	DEL18/21b/24
8	10	1.75	WT	WT
9	80	60	WT	WT
10	10	6.56	WT	WT
11	50	5.69	WT	WT
12	40	16.2	L858R	L858R
13	30	2.3	WT	WT
14	15	12.9	WT	WT
15	25	6.33	DEL19	DEL15
16	50	16	WT	WT
17	80	8.8	WT	WT
18	25	5.71	WT	WT
19	40	16.9	DEL19	DEL15
20	5	26.3	WT	WT
21	60	11.3	DEL19	DEL15
22	25	4.79	WT	WT
23	25	4.96	WT	WT
24	20	21.2	WT	WT
25	60	26.1	L858R	L858R
26	50	8.3	DEL19	DEL18/21b/24
27	30	7.05	WT	WT
28	50	1.99	WT	WT
29	25	5.86	WT	WT
30	25	0.5	WT	INVALID
31	50	15.3	DEL19	DEL15
32	30	10.9	WT	G719A
33	50	16.8	WT	WT
34	5	3.5	WT	DEL15
35	50	5.21	DEL19	DEL9/21a
36	50	6.14	WT	WT
37	60	13.7	WT	WT
38	25	3.71	WT	WT
39	25	1.58	L858R	L858R
40	10	4.08	WT	DEL15
41	30	8.61	WT	WT
42	20	7	WT	WT
43	50	4.57	WT	WT
44	5	14.7	WT	WT
45	5	0.28	WT	WT
46	20	1.21	WT	WT
47	20	2.65	WT	WT
48	50	3	DEL19	DEL15
49	70	6.5	DEL19	DEL15
50	25	9	L858R	L858R
51	50	5.19	DEL19	DEL15
52	50	10	DEL19	DEL15
53	70	5.7	DEL19	DEL9/21a
54	30	2.6	DEL19	DEL12
55	30	2.7	DEL19	DEL15
56	50	0.2	L858R	L858R
57	30	5.2	DEL19	DEL15
58	5	1.21	DEL19	DEL15
59	20	1.65	WT	WT
60	60	9.07	WT	INVALID
61	50	36.4	WT	WT
62	30	2.87	WT	G719C
63	30	1.29	DEL19	DEL15
64	5	3.02	DEL19	DEL15
65	50	20.8	DEL19	DEL15
66	40	22.4	L858R	L858R
67	50	2.63	DEL19	DEL9/21a
68	20	5	DEL19	DEL9/21a
69	20	60	WT	WT
70	25	4.97	WT	WT
71	50	5.15	DEL19	DEL15
72	25	8	DEL19	DEL15
73	40	60	WT	WT
74	30	20.8	WT	WT
75	50	0.5	WT	WT
76	10	5.4	WT	WT

A series of 76 archival DNAs extracted from lung adenocarcinoma cytological slides is reported. In all cases, the neoplastic cell percentage and the DNA concentration (ng/µL) were assessed. EGFR mutational status, previously tested by our laboratory reference method (fragment length and TaqMan assays), was re-evaluated by Idylla. DEL15, DEL18/21b/24, DEL9.21a: Idylla result reporting the different families of EGFR exon 19 deletions.
EGFR, epidermal growth factor receptor.