List of PCR primers used for the optimisation of mHRM analysis
| Gene | Primer sequence | Product size (bp) | Predicted Tm (°C) |
|---|---|---|---|
| TP53 E8b | F: GCCTGTCCTGGGAGAGAC | 53 | 84.3 |
| R: CCTTTCTTGCGGAGATTCT | |||
| TP53 E4d | F: GGCAGCTACGGTTTCC | 80 | 87.8 |
| R: CCCTCAGGGCAACTGA | |||
| SMAD4 E9a | F: GTATTGGTGTTCCATTGCTTACT | 56 | 76.9 |
| R: TAAATGTCTCTCCTACCTGAACAT |
mHRM, multiplex high-resolution melting; Tm, melting temperature.