Antibody | 1960 | 1970 | 1980 | 1990 | 2000 | 2010 |
Ki67 | +/− | -/− | +/− | +/− | +/+ | +/+ |
+/− | +/− | +/+ | +/− | +/− | +/+ | |
+/− | −/− | +/+ | +/− | +/+ | +/+ | |
CD31 | +/+ | −/− | +/− | +/+ | +/+ | +/+ |
+/+ | −/− | +/+ | +/− | +/+ | +/+ | |
+/+ | −/− | +/+ | +/+ | +/+ | +/+ | |
CD34 | +/+ | −/− | +/+ | +/+ | +/+ | +/+ |
+/+ | +/− | +/+ | +/+ | +/+ | +/+ | |
+/− | +/+ | +/+ | +/+ | +/+ | +/+ | |
LCA | +/− | −/− | +/+ | +/+ | +/+ | +/+ |
+/+ | +/− | +/+ | +/+ | +/+ | +/+ | |
+/+ | +/− | +/+ | +/+ | +/+ | +/+ | |
Ck 5 & 6–7 - AE1–AE3 –CAM 5.2; des; S-100; SMA; vim | +/+ | +/+ | +/+ | +/+ | +/+ | +/+ |
+/+ | +/+ | +/+ | +/+ | +/+ | +/+ | |
+/+ | +/+ | +/+ | +/+ | +/+ | +/+ |
+/+ intensity graded equal to positive control; +/− intensity graded lower than positive control; −/− no appropriate staining present.
Ck, cytokeratin; des, desmin; LCA, leucocyte common antigen; SMA, smooth muscle actin; vim, vimentin.