PAX8* | WT1† | ER | p53 | ARID1A | β-cat | calr | CK7 | CK20 | CEA | Specific | |
Primary ovarian tumours | |||||||||||
HGSC | + | + | + | M | wt | wt | + | − | − | p16 | |
LGSC | + | + | + | wt | wt | wt | −/+ | + | − | − | KRAS mut, BRAF mut |
EMC | + | − | + | wt>M | M>wt | M>wt | − | + | − | − | PTEN and MMR loss Presence of endometriosis |
CCC | + | − | −/+ | wt | M>wt | wt | + | − | − | Napsin A, HNF1β, AMACR | |
MC | + | − | − | wt>M | wt | wt | − | + | + | −/+ | Presence of teratoma or Brenner tumour |
SMT | + | − | + | wt | M>wt | wt | − | + | − | − | Presence of endometriosis |
GCT/SCST | −/+ | + | +/− | wt | wt | wt | + | − | − | − | Inhibin, SF1, FOXL2 mut (GCT), DICER1 mut (SCST), EMA |
SCC-HT | + | + | − | M | wt | wt | + | − | − | − | SMARCA4 (BRG1) loss |
Metastatic adenocarcinomas | |||||||||||
Colorectal | − | − | − | wt>M | wt | M>wt | − | − | + | + | CDX2, SATB2 |
Biliopancreatic | − | − | − | M>wt | wt | wt | − | + | + | + | SMAD4 (DPC4) loss |
Breast | − | − | +/− | wt>M | wt | wt | − | + | − | − | GCDFP15, mammaglobin |
Lung | − | − | − | wt>M | wt | wt | − | + | − | − | TTF1, Napsin A |
Kidney | + | + | − | wt | wt | wt | − | −/+ | − | − | CD10, vimentin, HIF1β |
Endocervix | + | − | −/+ | wt>M | wt | wt | − | + | − | + | p16, HPV |
Uterine EMC | + | − | + | wt>M | M>wt | M>wt | − | + | − | − | Vimentin, PTEN and MMR loss |
USC | + | −/+ | −/+ | M | wt | wt | − | + | − | − | Vimentin |
ARID1A M consists in the loss of IHC staining, while ARID1A wt is nuclear staining. β-cat M is nuclear positivity; β-cat wt is cytoplasm, membrane positivity or negativity. p53 M corresponds to intense and diffuse positivity in ≥60% of cells or complete negativity; p53 wt is the presence of rare cells weakly positive or positivity in <60% of cells.
*PAX8 is also expressed in kidney, thyroid, parathyroid and thymic carcinomas.
†WT1 stains also normal mesothelium, kidney, their derived neoplasias and desmoplastic small round cell tumour.
β-cat, β-catenin; calr, calretinin; CCC, clear cell carcinoma; CK, cytokeratin; EMC, endometrioid carcinoma; GCT/SCST, granulosa cell tumour/sex-cord stromal tumour; HGSC, high-grade serous carcinoma; LGSC, low-grade serous carcinoma; M, mutant pattern; MC, mucinous carcinoma; MMR, DNA mismatch repair genes; mut, mutation; SCC-HT, small cell carcinoma of hypercalcaemic type; SMT, seromucinous tumour; USC, uterine serous carcinoma; wt, wild-type pattern.