Details of antibodies used for immunohistochemistry (IHC) of triple-negative breast cancer sections
| Antibody | Clone | Dilution | Source | Antigen retrieval | Cellular localisation |
| ER | SP1 | 1:50 | Thermo Scientific Lab Vision RM9101-S | TE | Nucleus |
| PR | SP2 | 1:200 | Thermo Scientific Lab Vision RM9102-S | TE | Nucleus |
| HER2 | SP3 | 1:200 | Thermo Scientific Lab Vision RM9103-S | TE | Cytoplasmic/membrane |
| CK14 | LL002 | 1:20 | Leica Novocastra NCL-L-LL002 | TE | Cytoplasm |
| EGFR | E30 | 1:50 | Dako M7239 | Pro K | Cytoplasmic/membrane |
| Cytokeratin high molecular weight | 34βE12 | 1:20 | Dako M0630 | TE | Cytoplasm |
| Caveolin-1 | Polyclonal | 1:150 | Santa Cruz sc-894 | NT | Cytoplasm for stromal cells; cytoplasm/membrane for tumour cells |
EnVision Detection System (Dako K5007) was used for all IHC, except for caveolin-1 (Bond Polymer Refine Detection; Leica Novocastra DS9800).
NT, no treatment; Pro K, proteinase K, room temp, 10 min; TE, 0.01M Tris–EDTA pH 9.0, 98°C, 15 min.