Table 2

Primer sequences used for screening and sequencing

PrimerSequence 5’−3’Length
(bp)
GC 
(%)
Tm
(°C)
E1FGCCATCTACAAGCAGTCACA2050.0057.90
I1RGCAATCAGTGAGGAATCAGAGG2250.0058.80
I1FCACTGATTGCTCTTAGGTCTG2147.6256.08
E2RCATAGGGCACCACCACACTA2055.0059.09
E3FTGGCTCTGACTGTACCACCA2055.0060.47
E3RGGAGTCTTCCAGTGTGATGATG2250.0058.47
I2FseqCCTGCTTGCCACAGGTCT1861.1159.57
I3RseqTGGAAGAAATCGGTAAGAGGTG2245.4557.80
I4Rseq*GTTGGGCAGTGCTCGCTTAG2060.0061.64
  • F indicates a forward primer and R a reverse primer. Primers marked seq were used for sequencing.

  • GC, guanine and citosine.

  • *Spans junction between exons 4 and 5.