Primer | Sequence 5’−3’ | Length (bp) | GC (%) | Tm (°C) |
E1F | GCCATCTACAAGCAGTCACA | 20 | 50.00 | 57.90 |
I1R | GCAATCAGTGAGGAATCAGAGG | 22 | 50.00 | 58.80 |
I1F | CACTGATTGCTCTTAGGTCTG | 21 | 47.62 | 56.08 |
E2R | CATAGGGCACCACCACACTA | 20 | 55.00 | 59.09 |
E3F | TGGCTCTGACTGTACCACCA | 20 | 55.00 | 60.47 |
E3R | GGAGTCTTCCAGTGTGATGATG | 22 | 50.00 | 58.47 |
I2Fseq | CCTGCTTGCCACAGGTCT | 18 | 61.11 | 59.57 |
I3Rseq | TGGAAGAAATCGGTAAGAGGTG | 22 | 45.45 | 57.80 |
I4Rseq* | GTTGGGCAGTGCTCGCTTAG | 20 | 60.00 | 61.64 |
F indicates a forward primer and R a reverse primer. Primers marked seq were used for sequencing.
GC, guanine and citosine.
*Spans junction between exons 4 and 5.