Table 3

Clinicopathological, molecular and phenotypic characteristics of cases with different TCR-GR patterns

ClonalPolyclonalPCPP values
Age mean (SE)
(range)
56.29 (3.8)
(31-88)
50.07 (1.3)
(19-86)
50.88 (2.8)
(19-81)
0.3676
Histology0.4380
  VA9/17 (53%)65/161 (40%)17/43 (40%)
 ↑IELs without VA5/17 (29%)38/161 (24%)14/43 (32%)
 Normal3/17 (18%)58/161 (36%)12/43 (28%)
Positive serology7/14 (50%)57/141 (40%)15/42 (36%)0.7373
Diarrhoea9/17 (53%)97/163 (60%)23/43 (53%)0.7091
Immunomodulatory therapy5/17 (29%)32/149 (21%)7/36 (19%)0.7031
TCR PCR primer sets0.5081*
 D–J5/17 (30%)0/16319/43 (44%)
 V–J6/17 (35%)0/16310/43 (23%)
 D–J and V–J6/17 (35%)0/16314/43 (33%)
Flow (%) mean (SE)(range)
 CD8(+)56.35 (7) (2–90)60.99 (1.2) (5–94)59.37 (2.5) (13–82)0.5259
 CD4(+)13.94 (3.6) (4–57)15.46 (1.0) (2–82)16.28 (1.8) (4–67)0.8022
 TCR a/b62.82 (6.2) (10–90)64.52 (1.1) (33–93)63.76 (2.5) (13–92)0.8929
 TCR g/d13.06 (2.9) (1–38)20.37 (1.1) (1–62)19.17 (2.3) (1–56)0.1359
 TCR(-)0.47 (2.0) (−23 to 18)3.14 (0.5) (−12 to 43)4.24 (1.2) (−23 to 29)0.1630
 sCD3(-)17.8 (8.2) (−2 to 86)2.77 (0.4) (−14 to 24)3.41 (1.9) (−12 to 70)<0.0001 (0.0227†, 0.6141‡)
  • *Analysis of clonal and PCP groups only.

  • †Analysis excluding the RCDII group.

  • ‡Analysis of polyclonal and PCP groups only.

  • GR, gene rearrangement; IELs, intraepithelial lymphocytes; PCP, prominent clonal peaks; RCDII, refractory coeliac disease type II; TCR, T cell receptor rearrangement; VA, villous atrophy; SE, standard error.