Clonal | Polyclonal | PCP | P values | |
Age mean (SE) (range) | 56.29 (3.8) (31-88) | 50.07 (1.3) (19-86) | 50.88 (2.8) (19-81) | 0.3676 |
Histology | 0.4380 | |||
VA | 9/17 (53%) | 65/161 (40%) | 17/43 (40%) | |
↑IELs without VA | 5/17 (29%) | 38/161 (24%) | 14/43 (32%) | |
Normal | 3/17 (18%) | 58/161 (36%) | 12/43 (28%) | |
Positive serology | 7/14 (50%) | 57/141 (40%) | 15/42 (36%) | 0.7373 |
Diarrhoea | 9/17 (53%) | 97/163 (60%) | 23/43 (53%) | 0.7091 |
Immunomodulatory therapy | 5/17 (29%) | 32/149 (21%) | 7/36 (19%) | 0.7031 |
TCR PCR primer sets | 0.5081* | |||
D–J | 5/17 (30%) | 0/163 | 19/43 (44%) | |
V–J | 6/17 (35%) | 0/163 | 10/43 (23%) | |
D–J and V–J | 6/17 (35%) | 0/163 | 14/43 (33%) | |
Flow (%) mean (SE)(range) | ||||
CD8(+) | 56.35 (7) (2–90) | 60.99 (1.2) (5–94) | 59.37 (2.5) (13–82) | 0.5259 |
CD4(+) | 13.94 (3.6) (4–57) | 15.46 (1.0) (2–82) | 16.28 (1.8) (4–67) | 0.8022 |
TCR a/b | 62.82 (6.2) (10–90) | 64.52 (1.1) (33–93) | 63.76 (2.5) (13–92) | 0.8929 |
TCR g/d | 13.06 (2.9) (1–38) | 20.37 (1.1) (1–62) | 19.17 (2.3) (1–56) | 0.1359 |
TCR(-) | 0.47 (2.0) (−23 to 18) | 3.14 (0.5) (−12 to 43) | 4.24 (1.2) (−23 to 29) | 0.1630 |
sCD3(-) | 17.8 (8.2) (−2 to 86) | 2.77 (0.4) (−14 to 24) | 3.41 (1.9) (−12 to 70) | <0.0001 (0.0227†, 0.6141‡) |
*Analysis of clonal and PCP groups only.
†Analysis excluding the RCDII group.
‡Analysis of polyclonal and PCP groups only.
GR, gene rearrangement; IELs, intraepithelial lymphocytes; PCP, prominent clonal peaks; RCDII, refractory coeliac disease type II; TCR, T cell receptor rearrangement; VA, villous atrophy; SE, standard error.