Table 2

Detection of free intraperitoneal tumour cells based on conventional cytology, qRT-PCR for mRNA of CEA, EpCAM, CA-125 and CEA protein analysis at the first PIPAC procedure and at the third PIPAC procedure.

Sensitivity
(95% CI)
SpecificityTest positives PIPAC 1Test positives PIPAC 3
Conventional cytologyXX15/26 (58%)10/19 (53%)
mRNA CEA0.75 (0.53 to 0.90)1.0018/24 (75%)12/21 (57%)
mRNA EpCAM0.71 (0.49 to 0.87)1.0017/24 (71%)14/21 (67%)
mRNA CA-1250.43 (0.18 to 0.71)1.00XX
mRNA CEA/EpCAM0.88 (0.71 to 0.97)1.0020/24 (83%)16/21 (76%)
CEA proteinXX15/21 (71%)14/19 (74%)
  • The sensitivity and specificity of mRNA analyses are found by the receiver operating characteristics shown in figure 1.

  • CA-125, cancer antigen 125; CEA, carcinoembryonic antigen; EpCAM, epithelial cell adhesion molecule; PIPAC, pressurised intraperitoneal aerosol chemotherapy; X, not analysed; qRT-PCR, quantitative reverse transcriptase PCR.