TruSight Tumor26 NGS | Idylla BRAF | Idylla EGFR | Idylla KRAS | |
Turnaround time | 3 days | 90 min | 150 min | 120 min |
Hands-on time | 4 hours* | 5 min | 5 min | 5 min |
Mutations (n) | Hotspot regions (26 genes); small insertions/deletions, SNVs | 7 SNV | 51 SNV/del/ins | 21 SNV |
CE-IVD | No (RUO) | Yes | Yes | Yes |
Upfront extraction | Yes | No | No | No |
Minimal input: FFPE | 50 ng ≥10% tumour cells | 1×5 µm FFPE section between 50 and 600 mm²† and ≥50% tumour cells‡ | 1×5 µm FFPE section and ≥10% tumour cells‡ | 1×5 µm FFPE section between 50 and 600 mm²† and ≥10% tumour cells‡ |
Data processing | Open source | Closed source | Closed source | Closed source |
Required expertise | High | Low | Low | Low |
Output | VCF file | Mutation present: yes/no+specific or group of mutations | Mutation present: yes/no+specific or group of mutations | Mutation present: yes/no+specific or group of mutations |
*These are estimates based on the turnaround time at the University Hospitals of Leuven.
†Alternatively, a 1×10 µm FFPE section between 25 and 300 mm².
‡If less microdissection is necessary.
FFPE, formalin-fixed paraffin-embedded; RUO, research use only;SNV, single nucleotide variant; VCF, variant call format.