Table 1A

Summary table of in-house protocols published by public health and research labs at the time of discovery of COVID-19 (https://www.who.int/emergencies/diseases/novel-coronavirus-2019/technical-guidance/laboratory-guidance)

CountryInstituteGene targetsReference
ChinaChina CDCORF1ab and N http://ivdc.chinacdc.cn/kyjz/202001/t20200121_211337.html
GermanyCharitéRdRP, E, N https://www.who.int/docs/default-source/coronaviruse/protocol-v2-1.pdf?sfvrsn=a9ef618c_2
Hong Kong SARHKUORF1b-nsp14, N https://www.who.int/docs/default-source/coronaviruse/peiris-protocol-16-1-20.pdf?sfvrsn=af1aac73_4
JapanNational Institute of Infectious Diseases, Department of Virology IIIPancorona and multiple targets, spike protein https://www.who.int/docs/default-source/coronaviruse/method-niid-20200123-2.pdf?sfvrsn=fbf75320_7
ThailandNational Institutes of HealthN https://www.who.int/docs/default-source/coronaviruse/conventional-rt-pcr-followed-by-sequencing-for-detection-of-ncov-rirl-nat-inst-health-t.pdf?sfvrsn=42271c6d_4
USA*US CDCThree targets in N gene https://www.fda.gov/media/134922/download
FranceInstitut Pasteur, ParisTwo targets in RdRP https://www.who.int/docs/default-source/coronaviruse/real-time-rt-pcr-assays-for-the-detection-of-sars-cov-2-institut-pasteur-paris.pdf?sfvrsn=3662fcb6_2
  • *CDC update effective from 15 March 2020.

  • CDC, Centers for Disease Control and Prevention; ORF, open reading frame.