Mean Ct and per cent positivity of a dilution series of samples spiked with in-vitro transcribed RNA of the entire N-gene performed in triplicate are shown for SARS-CoV-2 N1 and N2 targets and a human RNase P control. Concentrations of the IVT RNA dilution series are reported as genomes per microlitre. Results indicate that the lowest concentration at which 100% of samples run in triplicate were detected for both N1 and N2 targets of SARS-CoV-2 genome was 3.3 genomes per microlitre. RNase P primers were used as a positive control to ensure proper specimen collection and monitor against substantial extraction, PCR inhibition, or reagent failure.