Days | E gene | RdPR gene | N gene |
0 | 37.06 | 37.65 | 33.43 |
20 | 37.68 | ND | 34.61 |
27 | ND | ND | 36.24 |
30 | 39.08 | ND | 36.92 |
36 | 36.42 | 35.06 | 39.08 |
42 | 40.8 | ND | 40.8 |
48 | ND | ND | ND |
The swabs were submitted to thermolysis (15 µL of samples mixed with 45 µL of distilled water protocol: 98°C for 3′ and 4°C for 5′). Then, 5 µL were tested with the Allplex SARS-CoV-2 assay (Seegene, Seoul, South Korea) on the CFX Connect Real-Time PCR Detection System (Bio-rad, Hercules, California, USA) following manufacturer’s instructions.
The cycle threshold for E, RdPR and N genes are displayed.
ND, not detected.