Table 1

Antibodies used in this study

AntigenAntibody or kitDilutionAntigen retrieval methodsSupplier
ERMonoclonal, 1D5PredilutedWater bath, 99°C, 40 minNichirei (Tokyo, Japan)
PgRmouse monoclonal, PgR636×40Water bath, 99°C, 40 minThermo Fisher Scientific (Waltham, Massachusetts, USA)
HER2HercepTestKitWater bath, 99°C, 20 minDAKO (Glostrup, Denmark)
Ki-67Monoclonal, MIB-1×100Microwave treatment for 15 minDAKO (Glostrup, Denmark)
CD98hcPolyclonal, H300×200not doneSanta Cruz biotechnology (Santa Cruz, California, USA)
LAT1Monoclonal, 4A2PredilutedMicrowave treatment for 15 minJ Pharma (Yokohama, Japan)
  • ER, estrogen receptor; HER2, human epidermal growth factor receptor-2; LAT1, L-type amino acid transporter 1; PgR, progesterone receptor.