Statistical analysis of the cycle thresholds
| CV | R2 | ||
| Fusion | |||
| IC | 20°C | 0.921 | 0.0117 |
| 4°C | 1.423 | 0.00834 | |
| Target | 20°C | 1.014 | 0.220 |
| 4°C | 0.599 | 0.0535 | |
| m2000 | |||
| IC | 20°C | 2.924 | 0.022 |
| 4°C | 5.521 | 0.144 | |
| Target | 20°C | 16.362 | 0.192 |
| 4°C | 17.55 | 0.139 | |
| ARIES | |||
| IC | 20°C | 2.482 | 0.360 |
| 4°C | 2.323 | 0.0551 | |
| Target N | 20°C | 2.812 | 0.406 |
| 4°C | 3.525 | 0.343 | |
| Target ORF | 20°C | 2.558 | 0.488 |
| 4°C | 3.794 | 0.390 |
CV, coefficient of variation; IC, internal amplification control; ORF, open-reading frame.