Cycle threshold (Ct) values of the individual and pool samples for the validation and study groups
| Pool size | Pool sample Ct target E | Individual sample Ct target E | Pool sample Ct target N2 | Individual sample Ct target N2 |
| Validation samples* | ||||
| 5 | 29.5 | 27.7 | 33.4 | 30.6 |
| 5 | 30.0 | 26.6 | 33.0 | 29.5 |
| 5 | 29.7 | 26.3 | 32.5 | 27.6 |
| 5 | 29.7 | 26.6 | 32.8 | 29.2 |
| 5† | 14.6 | 13.3 | 17.5 | 15.7 |
| 5† | 15.1 | 13.3 | 17.5 | 15.7 |
| 5† | 15.4 | 13.3 | 17.9 | 15.7 |
| 5 | 30.7 | 29.2 | 33.7 | 31.5 |
| 5 | 32.8 | 29.8 | 36.3 | 33.2 |
| 5† | 30.0 | 28.4 | 32.9 | 30.7 |
| 5† | 30.3 | 28.4 | 33.3 | 30.7 |
| 5† | 30.7 | 28.4 | 33.7 | 30.7 |
| 5 | 32.8 | 29.3 | 36.1 | 32.3 |
| Study samples with positive pool results | ||||
| 4 | 36.0 | 34.7 | 39.1 | 37.4 |
| 4 | 0 | 42.3 | 40.2 | 38.5 |
| 3 | 32.3 | 30.6 | 34.7 | 33.3 |
| 5‡ | 0 | 0 | 38.7 | 0 |
| 4 | 0 | 0 | 38.5 | 39.2 |
| 5 | 0 | 40.9 | 40.3 | 38.8 |
| 3‡ | 0 | 0 | 42.9 | 0 |
| 5 | 0 | 35.5 | 40.3 | 37.4 |
| 5 | 0 | 0 | 42.2 | + |
| 5 | 35.3 | +§ | 38.1 | + |
| 5‡ | 0 | 0 | 41.4 | 0 |
| 5 | 0 | 0 | 41.6 | 40.6 |
| 5 | 36.7 | + | 37.2 | + |
| 5‡ | 0 | 0 | 43.4 | 0 |
| 5 | 35.9 | + | 40.3 | + |
| 5 | 36.2 | + | 39.5 | + |
| 5 | 0 | 0 | 43.7 | + |
| 4‡ | 38 | 0 | 39.9 | 0 |
| 2‡ | 0 | 0 | 42.7 | 0 |
| 5 | 0 | 35.8 | 41.6 | 38.0 |
| 5 | 38.0 | 35.3 | 39.4 | 36.5 |
| 4 | 40.9 | 37.7 | 39.1 | 38.4 |
| 4 | 0 | 0 | 43.8 | 40.4 |
| 5 | 25.9 | 23.8 | 28.1 | 25.8 |
| 5 | 0 | 0 | 41.2 | 38.0 |
| 5 | 26.6 | 24.5 | 29.1 | 26.8 |
| 5‡ | 39.5 | 0 | 38.0 | 0 |
| 5 | 27.6 | + | 29.9 | + |
| 5‡ | 0 | 0 | 42.7 | 0 |
| 5‡ | 0 | 0 | 43.1 | 0 |
| 5 | 0 | 0 | 40.4 | 38.7 |
| 4‡ | 0 | 0 | 44.3 | 0 |
| 5 | 42.5 | 41.1 | 0 | 0 |
| 4‡ | 43.7 | 0 | 40.7 | 0 |
| 5 | 28.1 | 26.2 | 30.4 | 28.9 |
*Only 9 of 15 positive validation samples with available Ct values were included in the table.
†Validation samples tested thrice to evaluate the reproducibility of the pooling method.
‡Discordant study samples showing positive pool tests and negative individual tests. The negative results of all the individual samples were confirmed at an outside laboratory.
§Ct values are not generated for positive samples with ‘+’ sign.
E, envelope; N2, nucleic acid.