Tumours associated with H3.3 aberrations
| Mutation | Gene | Immunohistochemistry | |
| Chondroblastoma | p.Lys36Met |
H3F3B (95%) H3F3A (5%) | H3 K36M |
| Giant cell tumour of bone | p.Gly34Trp | H3F3A | H3 G34W |
| Diffuse midline glioma, H3 K27-altered | p.Lys27Met |
H3F3A
HIST1H3B/C | H3 K27M |
| Diffuse hemispheric glioma, H3 G34-mutant | p.Gly34Arg/Val | H3F3A | H3 G34R/V |
| Malignant peripheral nerve sheath tumour | Loss of trimethylation |
EED
SUZ12 | H3K27me3 (loss) |