FFPE OPA performance metrics | |
Metric | Target |
Final reads | 10–12M |
Raw read accuracy | 98–99% |
% Loading | 87%–92% |
Enrichment | 99.90% |
Library | 99.90% |
Mapped reads/DNA library | > 500K (>800K for 5% LOD) |
Mapped reads/RNA library | > 100K |
% Reads on target | >90% |
Base coverage depth | >1000 (>2500 for 5% LOD) |
Uniformity | 97%–99% (>90% for 90%) |
End-to-end reads | >90% |
Reads/amplicon | >500 |
AF | > 5%/0.05 |
MAPD | >0.5 (0.18–0.24) |
RNA detection | >5/7 |
Mean AQ20 read length | 85–95 |
Mean read length DNA | 85–100 |
Mean read length RNA | 70–100 |
Base call accuracy | 97%–99% |
AF, allelic fraction; LOD, limits of detection; MAPD, median absolute pairwise difference.