Table 1

Decalcification protocols for HCl and EDTA

Decalcification solution
HCl/conventional decalcification solutionEDTA decalcification solution (PH 7.2–7.4)
Hydrochloric acid5% (mL)Distilled water66.7% (mL)
Formic acid10% (mL)NaOH4.25% (g)
Acetic acid2.5% (mL)EDTA5% (g)
95% ethanol16% (mL)PBS33.3% (mL)
10% neutral formalin66.5% (mL)

  • HCl, hydrochloric acid; PBS, phosphate buffer saline.