Specificity and sensitivity of p53 immunohistochemistry in distinguishing NDBE biopsies from progressor from non-progressor patients
| p53 staining parameters | Cut-off points | Non-progressor versus progressor BE | |||
| Sensitivity | Specificity | PPV | NPV | ||
| 3+p53 positive columnar cells (automated) | (≥10 cells) | 39% | 93% | 82% | 66% |
| 3+p53 positive columnar cells (manual) | (≥10 cells) | 46% | 95% | 88% | 70% |
| 3+p53 positive gland | (≥1 gland) | 27% | 97% | 89% | 63% |
| 2+p53 positive columnar cells | (≥150 cells) | 70% | 69% | 64% | 75% |
| 3+p53 positive surface epithelium | Present/absent | 6% | 100% | 100% | 58% |
| 3+p53 positive columnar cells (≥10) OR 3+p53 positive gland (≥1) OR 3+p53 surface epithelium (present or absent) | Present/absent | 48% | 87% | 73% | 68% |
BE, Barrett’s oesophagus; NDBE, non-dysplastic BE; NPV, Negative predictive value; PPV, Positive predictive value.