Comparison of p53 expression in NDBE biopsies at 1-year, 3-year and 5-year intervals between an index NDBE biopsy and advanced dysplasia diagnosis
| p53 staining parameters | NDBE progressor biopsies (n=115) | P value | |
| > 1 year (incident dysplasia) (n=87) | ≤ 1 year (prevalent dysplasia) (n=28) | ||
| 3+p53 positive columnar cells (mean±SD, median, range) | 173±717, 5, 0–5661 | 182±349, 4, 0–1357 | 0.294 |
| 3+p53 positive columnar cells | |||
| <10 cells | 54 (62%) | 16 (57%) | 0.642 |
| ≥10 cells | 33 (38%) | 12 (43%) | |
| 3+p53 positive glands (semi-quantitative) (mean±SD, median, range) | 8±54, 0, 0–500 | 14±41, 0, 0–200 | 0.504 |
| p53 positive glands | 0.825 | ||
| ≥1 3+ positive gland | 23 (26%) | 8 (29%) | |
| No 3+positive gland | 64 (74%) | 20 (71%) | |
| 3+p53 positive surface epithelium | 4 (4.6%) | 3 (10.7%) | 0.239 |
| 3+p53 positive columnar cells (≥10 cells) OR any 3+p53 positive glands OR 3+p53 surface epithelium | 41 (47%) | 15 (54%) | 0.553 |
| p53 staining parameters | NDBE progressor biopsies (n=115) | P value | |
| >3 years (n=46) | ≤3 years (n=69) | ||
| 3+p53 positive columnar cells (mean±SD, median, range) | 214±891, 4, 0–5661 | 150±413, 5, 0–2784 | 0.513 |
| 3+p53 positive columnar cells | |||
| <10 cells | 29 (63%) | 41 (59.4%) | 0.697 |
| ≥10 cells | 17 (37%) | 28 (40.6%) | |
| 3+p53 positive glands (semiquantitative) (mean±SD, median, range) | 13±74, 0, 0–500 | 7±28, 0, 0–200 | 0.872 |
| p53 positive glands | |||
| ≥1 3+ positive gland | 12 (26%) | 19 (28%) | 0.864 |
| No 3+ positive gland | 34 (74%) | 50 (72%) | |
| 3+p53 positive surface epithelium | 1 (2.2%) | 6 (8.7%) | 0.152 |
| 3+p53 positive columnar cells (≥10 cells) OR any 3+p53 positive glands OR 3+p53 surface epithelium | 18 (39.1%) | 38 (55.1%) | 0.094 |
| p53 staining parameters | BE progressor biopsies (n=115) | P value | |
| >5 years (n=34) | ≤5 years (n=81) | ||
| 3+p53 positive columnar cells (mean±SD, median, range) | 111±403, 2, 0–2323 | 202±723, 7, 0–5661 | 0.118 |
| 3+p53 positive columnar cells | 0.335 | ||
| <10 cells | 23 (67.6%) | 47 (58.0%) | |
| ≥10 cells | 11 (32.4%) | 34 (42.0%) | |
| 3+p53 positive glands (semiquantitative) (mean±SD, median, range) | 2.9±7.8, 0, 0–40 | 12.6±60, 0, 0–500 | 0.643 |
| p53 positive glands | 0.592 | ||
| ≥1 3+ positive gland | 8 (23.5%) | 23 (28.4%) | |
| No 3+ positive gland | 26 (76.5%) | 58 (71.6%) | |
| 3+ p53 positive surface epithelium | 1 (2.9%) | 6 (7.4%) | 0.361 |
| 3+p53 positive columnar cells (≥10 cells) OR any 3+ p53 positive glands OR 3+ p53 surface epithelium | 11 (32.4%) | 45 (55.6%) | 0.023 |
BE, Barrett’s oesophagus; NDBE, non-dysplastic BE.