Quantitative analysis of p53 immunostaining in BE progressor versus BE non-progressor
| p53 staining parameters (N=285) | NDBE non-progressor biopsies (n=148) | NDBE progressor biopsies (n=115) | OR (95% CI) | P value |
| 3+p53 positive columnar cells (mean±SD, median, range) | 9.7±65.7, 1, 0–767 | 175.2±645.5, 4, 0–5661 | <0.001 | |
| 3+p53 positive columnar cells | <0.001 | |||
| < 10 cells | 138 (93%) | 70 (61%) | 8.9 | |
| ≥ 10 cells | 10 (7%) | 45 (39%) | (4.2 to 18.7) | |
| 3+p53 positive glands* (semiquantitative) (mean±SD, median, range) | 0.1±0.99, 0, 0–10 | 9.8±51.0, 0, 0–500 | <0.001 | |
| p53 positive glands | <0.001 | |||
| ≥1 3+ positive gland | 4 (3%) | 31 (27%) | 13.3 | |
| No 3+ positive gland | 144 (97%) | 84 (73%) | (4.5 to 38.9) | |
| 3+p53 positive surface epithelium | 0 | 7 (6%) | 0.003 | |
| 2+p53 positive columnar cells (mean±SD, median, range) | 151.0±220.2, 81, 0–1608 | 569.7±870.8, 295, 0–5092 | <0.001 | |
| 2+p53 positive columnar cells | <0.001 | |||
| <150 cells | 102 (69%) | 35 (30%) | 5.1 | |
| ≥150 cells | 46 (31%) | 80 (70%) | (3.0 to 8.6) | |
| 3+p53 positive columnar cells (≥10 cells) | ||||
| OR any 3+ p53 positive glands | 20 (14%) | 55 (48%) | 7.2 | <0.001 |
| OR 3+ p53 surface epithelium | (4.0 to 12.7) |
*A gland was defined as positive when ≥50% cells showed strong (3+) expression of p53.
BE, Barrett’s oesophagus; NDBE, non-dysplastic BE.