Biochemical and Biophysical Research Communications
Regular ArticleNongenomic Stimulation of Nitric Oxide Release by Estrogen Is Mediated by Estrogen Receptor α Localized in Caveolae☆
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Abbreviations used: E2, 17β-estradiol; E2BSA, 17β-estradiol 6-(O-carboxymethyl)oxime:BSA; E2BSA-FITC, 17β-estradiol 6-(O-carboxymethyl)oxime:BSA–fluorescein isothiocyanate conjugate; EC, endothelial cell; BAEC, bovine aortic endothelial cell; NO, nitric oxide; NOx, reduced NO−2 and NO−3; eNOS, endothelial nitric oxide synthase; ER, estrogen receptor; L-NAME, NG-nitro-l-arginine methyl ester; BAPTA-AM, 1,2-bis(2-aminophenoxy)-ethane-N,N,N′,N′-tetraacetic acid; tetrakis(acetoxymethyl ester; PBS, phosphate-buffered saline; HBSS, Hanks' balanced salt solution
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Present address: Lilly Korea Ltd., 906-4, Sang-shin-ri, Hyangnam–myun, Hwasung-kun, Kyunggi-do, Korea.
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To whom correspondence should be addressed. Fax: 82-2-388-0924. E-mail: [email protected].