Original PapersInsufficient Inactivation of HIV-1 in Human Cryo Poor Plasma by Beta-propiolactone: Results from a Highly Accurate Virus Detection Method
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Nef-Mediated CD3-TCR Downmodulation Dampens Acute Inflammation and Promotes SIV Immune Evasion
2020, Cell ReportsCitation Excerpt :The cell-associated virus load in peripheral blood was determined by a limiting dilution co-cultivation assay as described (Stahl-Hennig et al., 1992, 1996). Instead of testing supernatants of cell cultures for SIV p27, SIV positive cultures were identified by immunoperoxidase staining for intracellular viral proteins with a SIV-hyperimmune serum from an infected macaque as reported (Norley et al., 1993). To increase adherence of indicator cells, plates used for staining were coated with Concanavalin A (Gundlach et al., 1998).
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