Abstract
The HER2/neu gene (also known as ERBB2) is located on chromosome 17 (q11.2–q12) and encodes a glycoprotein known to be a member of the epidermal growth factor receptor family. Clinically, the determination of its amplification status is of utmost importance, as 10–35% of invasive human breast carcinomas come along with HER2/neu overexpression, and treatment has to be adjusted accordingly. Here a method to analyze HER2 FISH samples with digital microscopy, using the slide scanning platform Metafer PV (MetaSystems, Altlussheim, Germany), is presented. Metafer PV is a system for the automation of HER2/neu FISH assay analysis of samples hybridized with the Abbott™ PathVysion® probe kit.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Stevens, R., Almanaseer, I., Gonzalez, M., Caglar, D., Knudson, R.A., Ketterling, R.P., Schrock, D.S., Seemayer, T.A., and Bridge, J.A. (2007) Analysis of HER2 gene amplification using an automated fluorescence in situ hybridization signal enumeration system. Journal of Molecular Diagnostics 9, 144–50.
Dendukuri, N., Khetani, K., McIsaac, M., and Brophy, J. (2007) Testing for HER2-positive breast cancer: a systematic review and cost-effectiveness analysis. CMAJ 176, 1429–34.
Lörch, T., Piper, J., and Tomisek, J.D. (2002) “Tile Sampling”: a new method for the automated quantitative analysis of samples with high cell density and its application to HER2 scanning. In: Proceedings of the Third Euroconference on Quantitative Molecular Cytogenetics. Rosenön, Stockholm, Sweden.
Piper, J., Lörch, T., Poole, I., and Tomisek, J.D. (2002) Computing the HER2:CEP-17 ratio of tumour cells in breast cancer tissue sections by analysois of the FISH spot counts of a tiles sampling. In: Proceedings of the Third Euroconference on Quantitative Molecular Cytogenetics. Rosenön, Stockholm, Sweden.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2011 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Schunck, C., Mohammad, E. (2011). Automated Analysis of FISH-Stained HER2/neu Samples with Metafer. In: Al-Mulla, F. (eds) Formalin-Fixed Paraffin-Embedded Tissues. Methods in Molecular Biology, vol 724. Humana Press. https://doi.org/10.1007/978-1-61779-055-3_6
Download citation
DOI: https://doi.org/10.1007/978-1-61779-055-3_6
Published:
Publisher Name: Humana Press
Print ISBN: 978-1-61779-054-6
Online ISBN: 978-1-61779-055-3
eBook Packages: Springer Protocols