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Enhanced cellular proliferative activity and cell death in chronic thyroiditis and thyroid papillary carcinoma

  • Original Paper
  • Clinical Oncology
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Abstract

For the analysis of cellular proliferative activity and cell death in thyroid diseases, the Ki-67 labeling index, bcl-2 protein expression and cell death of follicular epithelia by immunohistochemistry and in situ DNA nick-end labeling methods were evaluated in normal thyroid tissues as well as in surgical specimens from cases of Hashimoto's disease (16 cases), focal lymphocytic thyroiditis (13 cases), Graves' disease (15 cases), follicular adenoma (20 cases) and papillary carcinoma (43 cases). Cellular proliferative activity and cell death were both enhanced in cases of thyroiditis, including Hashimoto's disease and focal lymphocytic thyroiditis. Thyroids from patients with follicular adenoma and papillary carcinoma also showed increased cellular proliferative activity and cell death. In addition, predominant high cellularity and partial loss of bcl-2 protein expression in papillary carcinoma suggested that the overgrowth and dedifferentiation were associated with malignancy.

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References

  • Allan DJ, Howell A, Roberts SA, Williams GT, Watson RJ, Coyne JD, Clarke RB, Laidlaw IJ, Potten CS (1992) Reduction in apoptosis relative to mitosis in histologically normal epithelium accompanies fibrocystic change and carcinoma of the premenopausal human breast. J Pathol 167:25–32

    PubMed  Google Scholar 

  • Arends MJ, McGregor AH, Wyllie AH (1994) Apoptosis is inversely related to necrosis and determines net growth in tumors bearing constitutively expressedmyc, ras, andHPV oncogenes. Am J Pathol 144:1045–1057

    PubMed  Google Scholar 

  • Barbareschi M, Girlando S, Mauri FM, Forti S, Eccher C, Mauri FA, Togni R, Palma PD, and Doglioni C (1994) Quantitative growth fraction evaluation with MIB1 and Ki67 antibodies in breast carcinomas. Am J Clin Pathol 102:171–175

    PubMed  Google Scholar 

  • Bhargava V, Kell DL, van de Rijn M, Warnke RA (1994) Bcl-2 immunoreactivity in breast carcinoma correlates with hormone receptor positivity. Am J Pathol 145:535–540

    PubMed  Google Scholar 

  • Bowen ID, Sarraf CE (1986) Kinetic studies on a murine sarcoma and an analysis of apoptosis. Br J Cancer 54:989–998

    PubMed  Google Scholar 

  • Bronner MP, Culin C, Reed JC, Furth EE (1995) Thebcl-2 protooncogene and the gastrointestinal epithelial tumor progression model. Am J Pathol 146:20–26

    PubMed  Google Scholar 

  • Brown DC, Gatter KC (1990) Monoclonal antibody Ki-67: its use in histopathology. Invited review. Histopathology 17:489–503

    Google Scholar 

  • Bursch W, Kleine L, Tenniswood M (1990) The biochemistry of cell death by apoptosis. Biochem Cell Biol 68:1071–1074

    PubMed  Google Scholar 

  • Gavrieli Y, Sherman Y, Ben-Sasson SA (1992) Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J Cell Biol 119:493–501

    PubMed  Google Scholar 

  • Gerdes J, Lemke H, Baisch H, Wacker HH, Schwab U, Stein H (1984) Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67. J Immunol 133:1710–1715

    PubMed  Google Scholar 

  • Gompel A, Sabourin JC, Martin A, Yaneva H, Audouin J, Decroix Y, Poitout P (1994) Bcl-2 expression in normal endometrium during the menstrual cycle. Am J Pathol 144:1195–1202

    PubMed  Google Scholar 

  • Haldar S, Negrini M, Monne M, Sabbioni S, Croce CM (1994) Down-regulation of bcl-2 by p53 in breast cancer cells. Cancer Res 54:2095–2097

    PubMed  Google Scholar 

  • Hedinger Chr, Sobin LH (1974) Histological typing of thyroid tumors. International histological classification no. 11. WHO, Geneva, pp 21–26

    Google Scholar 

  • Hockenbery DM, Zutter M, Hickey W, Nahm M, Korsmeyer SJ (1991) Bcl2 protein is topographically restricted in tissues characterized by apoptotic cell death. Proc Natl Acad Sci USA 88:6961–6965

    PubMed  Google Scholar 

  • Joensuu H, Pylkkanen L, Toikkanen S (1994) Bcl-2 protein expression and longterm survival in breast cancer. Am J Pathol 145:1191–1198

    PubMed  Google Scholar 

  • Kasagi N, Gomyo Y, Shirai H, Tsujitani S, Ito H (1994) Apoptotic cell death in human gastric carcinoma. Analysis by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling. Jpn J Cancer Res 85:939–945

    PubMed  Google Scholar 

  • Korsmeyer SJ (1992) Bcl-2 initiates a new category of oncogenes: regulators of cell death. Blood 80:879–886

    PubMed  Google Scholar 

  • Lazarus JH, Burr ML, McGregor AM, Weetman AP, Ludgate M, Woodhead JS, Hall R (1984) The prevalence and progression of autoimmune thyroid disease in the elderly. Acta Endocrinol 106:199–202

    PubMed  Google Scholar 

  • LeBrun DP, Warnke RA, Cleary ML (1993) Expression of bcl-2 in fetal tissues suggests a role in morphogenesis. Am J Pathol 142:743–753

    PubMed  Google Scholar 

  • Mcgurrin JF, Doria Jr MI, Dawson PJ, Karrison T, Stein HO, Franklin WA (1987) Assessment of tumor cell kinetics by immunohistochemistry in carcinoma of breast. Cancer 59:1744–1750

    PubMed  Google Scholar 

  • Novack DV, Korsmeyer SJ (1994) Bcl-2 protein expression during murine development. Am J Pathol 145:61–73

    PubMed  Google Scholar 

  • Okayasu I, Fujiwara M, Hara Y, Tanaka Y, Rose NR (1995) Association of chronic lymphocytic thyroiditis and thyroid papillary carcinoma: study of surgical cases of Japanese and white and African Americans. Cancer (in press)

  • Pietenpol JA, Papdopoulos N, Markowitz S, Willson JKV, Kinzler KW, Vogelstein B (1994) Paradoxical inhibition of solid tumor cell growth by bcl2. Cancer Res 54:3714–3717

    PubMed  Google Scholar 

  • Pilotti S, Collini P, Rilke F, Cattoretti G, Bo RD, and Pierotti MA (1994) Bcl-2 protein expression in carcinomas originating from the follicular epithelium of the thyroid gland. J Pathol 172:337–342

    PubMed  Google Scholar 

  • Rosenthal MJ, Hunt WC, Garry PJ, Goodwin JS (1987) Thyroid failure in the elderly. JAMA 258:209–213

    PubMed  Google Scholar 

  • Saegusa M, Takano Y, Wakabayashi T, Okayasu I (1995) Apoptosis and cell proliferation in human gastric carcinoma. Jpn J Cancer Res 86:743–748

    PubMed  Google Scholar 

  • Shaw P, Bovey R, Tardt S, Sahli R, Sordat B, Costa J (1992) Induction of apoptosis by wild-type p53 in a human colon tumor-derived cell line. Proc Natl Acad Sci USA 89:4495–4499

    PubMed  Google Scholar 

  • Shepherd NA, Richman PI, England J (1988) Ki-67 derived proliferative activity in colorectal adenocarcinoma with prognostic correlations. J Pathol 155:213–219

    PubMed  Google Scholar 

  • Shimizu T, Usuda N, Yamada T, Sujenoya A, Iida F (1993) Proliferative activity of human thyroid tumors evaluated by proliferative cell nuclear antigen/cylin immunohistochemical studies. Cancer 71:2807–2812

    PubMed  Google Scholar 

  • Sinicrope FA, Ruan SB, Cleary KR, Stephens LC, Lee JJ, Levin B (1995) Bcl-2 and p53 oncoprotein expression during colorectal tumorigenesis. Cancer Res 55:237–241

    PubMed  Google Scholar 

  • Toth K, Vaughan MMG, Slocum HK, Arredondo MA, Takita H, Baker RM, Rustum YM (1994) New immunohistochemical “sandwich” staining method formdr1 P-glycoprotein detection with JSB-1 monoclonal antibody in formalin-fixed, paraffin-embedded human tissues. Am J Pathol 144:227–236

    PubMed  Google Scholar 

  • Tunbridge WMG, Brewis M, French JM, Appleton D, Bird T, Clark F, Evered DC, Evancs JG, Hall R, Smith P, Stephenson J, Young E (1981) Natural history of autoimmune thyroiditis. Br Med J 282:258–262

    Google Scholar 

  • Weidner N, Moore DHII, Vartanian R (1994) Correlation of Ki-67 antigen expression with mitotic figure index and tumor grade in breast carcinomas using the novel “paraffin”-reactive MIB1 antibody. Hum Pathol 25:337–342

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Pathology, School of Medicine, Kitasato University, Kitasato 1-15-1, 228, Sagamihara, Kanagawa, Japan

    Isao Okayasu & Makoto Saegusa

  2. Department of Pathology, Japanese Red Cross Medical Center, Shibuya, Tokyo, Japan

    Mutsunori Fujiwara

  3. Department of Internal Medicine, Ohme Municipal General Hospital, Ohme, Tokyo, Japan

    Yoshihito Hara

  4. Department of Molecular Microbiology and Immunology, School of Hygiene and Public Health, Johns Hopkins University, Baltimore, Maryland, USA

    Noel R. Rose

Authors
  1. Isao Okayasu
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  2. Makoto Saegusa
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  3. Mutsunori Fujiwara
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  4. Yoshihito Hara
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  5. Noel R. Rose
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Okayasu, I., Saegusa, M., Fujiwara, M. et al. Enhanced cellular proliferative activity and cell death in chronic thyroiditis and thyroid papillary carcinoma. J Cancer Res Clin Oncol 121, 746–752 (1995). https://doi.org/10.1007/BF01213321

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  • DOI: https://doi.org/10.1007/BF01213321

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