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Clinical effect of recombinant human granulocyte colony-stimulating factor (rhG-CSF) on various types of neutropenia including cyclic neutropenia

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Biotherapy

Abstract

Recombinant human granulocyte colony-stimulating factor (rhG-CSF) was investigated for its clinical efficacy in the treatment of various types of neutropenia (3 cases with idiopathic neutropenia of suspected drug induction, 5 cases with idiopathic neutropenia of other origin, and 2 cases with cyclic neutropenia). Treatment with glycosylated rhG-CSF produced in the Chinese Hamster Ovary cells at dose levels of 2–5μg/kg/day caused rapid increases of neutrophil counts associated with an improvement of the infection. In cyclic neutropenia patients, marked reduction in the duration of the neutropenic period was observed with rhG-CSF administration started before the period. Intercurrent stomatitis, which occurred in 1 patient, was markedly milder as compared to a previous episode which occurred during an untreated neutropenic period.

The treatment of rhG-CSF was well tolerated and no adverse events were observed, nor was there any detectable anti-rhG-CSF antibody in any patients studied; hence the clinical use of rhG-CSF is considered to be safe.

These results suggest beneficial effects of rhG-CSF on the recovery of neutrophil counts in cyclic and other types of idiopathic neutropenias, as well as for the treatment of neutropenia-associated infection.

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References

  1. Nomura H, Imazeki I, Oheda M et al. Purification and characterization of human granulocyte colony stimulating factor (G-CSF), EMBO J 1986; 5: 871–6.

    PubMed  Google Scholar 

  2. Tamura M, Hattori K, Nomura H et al. Induction of neutrophilic granulocytosis in mice by administration of purified human native granulocyte colony-stimulating factor (G-CSF). Biochem Biophys Res Comm 1987; 142: 454–60.

    PubMed  Google Scholar 

  3. Asano S, Ono M. Human granulocyte colony-stimulating factor: its biological actions and clinical implication. Acta Haematol Jpn 1987; 50: 1550–6.

    Google Scholar 

  4. Nagata S, Tsuchiya M, Asano S et al. Molecular cloning and expression of cDNA for human granulocyte colonystimulating factor. Nature 1986; 319: 415–8.

    PubMed  Google Scholar 

  5. Nagata S, Tsuchiya M, Asano S et al. The chromosomal gene structure and two mRNA for human granulocyte colony-stimulating factor. EMBO J 1986; 3: 575–81.

    Google Scholar 

  6. Oheda M, Hase S, Ono M, Ikenaka T. Structures of the sugar chains of recombinant human granulocyte colonystimulating factor produced by chinese hamster ovary cells. J Biochem 1988; 103: 544–6.

    PubMed  Google Scholar 

  7. Souza LM, Boone TC, Gabrilove J et al. Recombinant human granulocyte colony-stimulating factor: effect on normal and leukemic myeloid cells. Science 1986; 232: 61–5.

    PubMed  Google Scholar 

  8. Kodo H, Tajika K, Takahashi S et al. Acceleration of neutrophilic granulocyte recovery in bone marrow transplanted patients by recombinant human granulocyte colony-stimulating factor administration. Lancet II, 1988: 38–9.

    Google Scholar 

  9. Bronchud MH, Dcraffe JH, Thatcher N et al. Phase I/II study of recombinant human granulocyte colony-stimulating factor in patients receiving intensive chemotherapy for small cell lung cancer. Br J Cancer 1987; 56: 809–13.

    PubMed  Google Scholar 

  10. Bonilla MA, Gillio AP, Ruggeiro M et al. Effect of recombinant human granulocyte colony-stimulating factor on neutropenia in patient with congenital agranulocytosis. N Engl J Med 1989; 320: 1574–80.

    PubMed  Google Scholar 

  11. Jakubowski AA, Souza LM, Kelly F et al. Effect of recombinant human granulocyte colony-stimulating factor in patient with idiopathic neutropenia. N Engl J Med 1989; 320: 38–42.

    PubMed  Google Scholar 

  12. Pisciotta AV. Immune and toxic mechanisms in druginduced agranulocytosis. Semin Hematol 1973; 10: 279–310.

    PubMed  Google Scholar 

  13. Watari K, Asano S, Shirafuji N et al. Serum granulocyte colony-stimulating factor levels in healthy volunteers and patients with various disorders as estimated by enzyme immunoassay. Blood 1989; 73: 117–22.

    PubMed  Google Scholar 

  14. Kyle RA, Linman JW. Chronic idiopathic neutropenia. A newly recognized entity? N Engl J Med 1968; 279: 1015–9.

    PubMed  Google Scholar 

  15. Rodgers GM, Schuman MA. Acquired cyclic neutropenia: successful treatment with prednisone. Am J Hematol 1982; 13: 83–9.

    PubMed  Google Scholar 

  16. Roozendaal KJ, Dicke KA, Boonzajer Flaes MS. Effect of oxymetholone on human cyclic hematopoiesis. Br J Haematol 1981; 47: 185–93.

    PubMed  Google Scholar 

  17. Lange RD. Cyclic hematopoiesis: human cyclic neutropenia. Exp Haematol 1983; 11: 435–51.

    Google Scholar 

  18. Hammond WP, Prince TH, Souza LM et al. Treatment of cyclic neutropenia with granulocyte colony-stimulating factor. N Eng J Med 1989; 320: 1306–11.

    Google Scholar 

  19. Vadhan-Raj S, Keating M, Lemaistre A et al. Effects of recombinant human granulocyte-macrophage colony-stimulating factor in patients with myelodysplastic syndromes. N Engl J Med 1987; 317: 1545–52.

    PubMed  Google Scholar 

  20. Antin JH, Smith BR, Holmes W et al. Phase I/11 study of recombinant human granulocyte-macrophage colony-stimulating factor in aplastic anemia and myelodysplastic syndrome. Blood 1988; 72: 705–13.

    PubMed  Google Scholar 

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Hirashima, K., Yoshida, Y., Asano, S. et al. Clinical effect of recombinant human granulocyte colony-stimulating factor (rhG-CSF) on various types of neutropenia including cyclic neutropenia. Biotherapy 3, 297–307 (1991). https://doi.org/10.1007/BF02221322

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  • DOI: https://doi.org/10.1007/BF02221322

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