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The influence of Lactobacillus acidophilus-derived surfactants on staphylococcal adhesion and biofilm formation

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Abstract

The ability of surfactants obtained from three Lactobacillus acidophilus strains to inhibit Staphylococcus aureus and S. epidermidis biofilms was evaluated. Their influence was determined on bacterial initial adhesion, biofilm formation and dispersal using MTT-reduction assay, confocal laser scanning microscopy and image PHLIP analysis. The number of adhering S. aureus and S. epidermidis cells after a 3-h co-incubation with biosurfactants was reduced by 5–56 % in a strain-and dose-dependent manner. S. epidermidis-and, to a lower extent, in S. aureus-biofilm formation was also inhibited in the presence of the tested surfactants. The addition of surfactants to preformed mature biofilms accelerated their dispersal, and changed the parameters of biofilm morphology. The L. acidophilus-derived surfactants inhibit bacterial deposition rate and biofilm development (and also its maturation) without affecting cell growth probably due to the influence on the cell-surface hydrophobicity of staphylococci.

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Abbreviations

LAA:

Lactobacillus acidophilus

SAU:

Staphylococcus aureus

SEP:

Staphylococcus epidermidis

BSA:

bovine serum albumin

CFU:

colony-forming unit(s)

CLSM:

confocal laser scanning microscopy

CSH:

cell-surface hydrophobicity

FITC:

fluorescein isothiocyanate

MRS:

De Man-Rogosa-Sharpe (broth)

MTT:

3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide

PBS:

phosphate buffered saline

PHLIP:

phobia laser scanning microscopy imaging processor

SAT:

salt-aggregation test

TSB:

tryptic-soy broth

TSBGlc:

TSB with 0.25 % d-glucose

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Authors and Affiliations

  1. Department of Immunology and Infectious Biology, Institute of Microbiology and Immunology, University of Łódź, 90-237, Łódź, Poland

    E. Walencka, B. Sadowska & B. Różalska

  2. Department of Industrial Microbiology and Biotechnology, Institute of Microbiology and Immunology, University of Łódź, 90-237, Łódź, Poland

    S. Różalska

Authors
  1. E. Walencka
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  2. S. Różalska
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  3. B. Sadowska
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  4. B. Różalska
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Correspondence to B. Różalska.

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Walencka, E., Różalska, S., Sadowska, B. et al. The influence of Lactobacillus acidophilus-derived surfactants on staphylococcal adhesion and biofilm formation. Folia Microbiol 53, 61–66 (2008). https://doi.org/10.1007/s12223-008-0009-y

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  • DOI: https://doi.org/10.1007/s12223-008-0009-y

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