Elsevier

Human Pathology

Volume 25, Issue 1, January 1994, Pages 42-46
Human Pathology

Original contribution
Multidirectional differentiation in the normal, hyperplastic, and neoplastic human prostate: Simultaneous demonstration of cell-specific epithelial markers

https://doi.org/10.1016/0046-8177(94)90169-4Get rights and content

Abstract

The prostatic epithelium is composed of three distinct cell populations: secretory luminal, basal, and endocrine-paracrine cells. It is currently unknown whether these basic epithelial cell types are related in a hierarchical pathway of differentiation or are independent and separate entities. In the present study we used double-label techniques for cell-specific markers to search for multidirectional differentiation in normal, hyperplastic, and neoplastic prostate tissue. In normal and hyperplastic conditions subsets of basal cells revealed synchronous expression of basal cell-specific cytokeratins and the prostate-specific antigen, indicating intermediate differentiation between basal and secretory luminal cell types. Furthermore, endocrine-paracrine cells of the closed type focally showed simultaneous expression of chromogranin A and basal cell-specific cytokeratins. These findings highlight the phenotypic plasticity of the basal cell layer in the human prostate. In prostatic adenocarcinoma co-expression of exocrine (prostate-specific antigen) and endocrine (chromogranin A) markers was detected frequently in subsets of malignant cells. Conversely, this amphicrine phenotype was rarely found in hyperplastic glands. The occurrence of multidirectional differentiation within the prostatic endocrine cell system may indicate that endocrine-paracine cells derive from pluripotent stem cells of endodermal origin. Furthermore, the phenotypic plasticity of basal cells suggests that this epithelial compartment houses stem cell populations that give rise to all epithelial cell lineages encountered in the normal, hyperplastic, and neoplastic human prostate.

References (40)

  • M Masai et al.

    Immunohistochemical study of androgen receptor in benign hyperplastic and cancerous human prostates

    Prostate

    (1990)
  • JA Ruizeveld de Winter et al.

    Androgen receptor heterogeneity in human prostatic carcinomas visualized by immunohistochemistry

    J Pathol

    (1990)
  • G Dhom et al.

    Histology and immunohistochemistry studies in prostate cancer

    Am J Clin Oncol

    (1988)
  • RB Nagle et al.

    Phenotypic relationships of prostatic intraepithelial neoplasia to invasive prostatic carcinoma

    Am J Pathol

    (1991)
  • H Okada et al.

    Keratin profiles in normal/hyperplastic prostates and prostate carcinoma

    Virchows Arch A Pathol Anat Histopathol

    (1992)
  • HF English et al.

    Response of glandular versus basal rat ventral prostatic epithelial cells to androgen withdrawal and replacement

    Prostate

    (1987)
  • JT Isaacs et al.

    Etiology and disease process of benign prostatic hyperplasia

    Prostate

    (1989)
  • CJ Fickinger

    Ultrastructural observations on the postnatal development of the rat prostate

    Z Zellforsch

    (1971)
  • GB Dermer

    Basal cell proliferation in benign prostatic hyperplasia

    Cancer

    (1978)
  • GT Bazer

    Basal cell proliferation and differentiation in regeneration of the rat ventral prostate

    Invest Urol

    (1980)
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