Proliferating cell nuclear antigen is required for DNA excision repair

doi:10.1016/0092-8674(92)90416-A

Cell

Volume 69, Issue 2, 17 April 1992, Pages 367-374

https://doi.org/10.1016/0092-8674(92)90416-A Get rights and content

Abstract

Fractionation of extracts from human cell lines allows nucleotide excision repair of damaged DNA to be resolved into discrete incision and polymerization stages. Generation of incised intermediates depends on the XP-A protein, a polypeptide that recognizes sites of damaged DNA, and on the human single-stranded DNA-binding protein HSSB. The proliferating cell nuclear antigen (PCNA) is required for the DNA synthesis that converts the nicked intermediates to completed repair events. This need for PCNA implies that repair synthesis is carried out by DNA polymerase δ or ε. The ability to visualize repair intermediates in the absence of PCNA facilitates dissection of the multiprotein reaction that leads to incision of damaged DNA in a major pathway of cellular defense against mutagens.

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Cell

ArticleProliferating cell nuclear antigen is required for DNA excision repair

Abstract

Mutat. Res.

FEBS Lett.

FEBS Lett.

Mutat. Res.

J. Biol. Chem.

Mutat. Res.

J. Biol. Chem.

J. Biol. Chem.

J. Biol. Chem.

J. Biol. Chem.

J. Biol. Chem.

J. Biol. Chem.

Mutat. Res.

J. Biol. Chem.

J. Biol. Chem.

Cell

Cyclin/PCNA is the auxiliary protein of DNA polymerase-δ

Nature

Requirement for the replication protein SSB in human DNA excision repair

Nature

DNA replication and UV-induced DNA repair synthesis in human fibroblasts are much less sensitive than DNA polymerase α to inhibition by butylphenyldeoxyguanosine triphosphate

Nucl. Acids Res.

Article
Proliferating cell nuclear antigen is required for DNA excision repair