Antimutagenicity of cell fractions of microorganisms on potent mutagenic pyrolysates

https://doi.org/10.1016/0165-1218(93)90003-VGet rights and content

Abstract

The inactivation of 3-amino-1,4-dimethyl-5 H-pyrido[4,3-b]indole (Trp-P-1) and 2-amino-6-methyldipyrido[1,2-a:3′,2′-d]imidazole (Glu-P-1) by binding of mutagenic pyrolysate to fractions of microorganisms (their desmutagenic and bio-antimutagenic activity) was investigated. All strains bound Trp-P-1 effectively, but Glu-P-1 to a lesser extent. The Gram-negative bacteria (GNB) could bind about 10–20 μg/mg of Trp-P-1 more than the Gram-positive bacteria (GPB), and about 50–60 μg/mg more than the yeasts. The cell wall skeletons of all strains tested had great binding ability, but in the cytoplasm of all strains tested it was lower. The peptidoglycan, outer membrane, and glucan isolate from the cell wall skeletons showed the highest binding ability to Trp-P-1. The cell wall skeletons of the tested strains greatly inhibited the mutagenicity induced by Trp-P-1, and to a lower extent that of 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx). Although the cultured broth and solution of cells extracted by phosphate buffer (pH 7.0) showed antimutagenicity against Trp-P-1, this activity was lower than the binding of Trp-P-1 to the cells. The cultured broth and freeze-dried cytoplasms of yeast cells showed bio-antimutagenicity towards Trp-P-1, but those of all bacteria tested did not.

Cited by (45)

  • A comparative study of procedures for binding of aflatoxin M1 to Lactobacillus rhamnosus GG

    2018, Brazilian Journal of Microbiology
    Citation Excerpt :

    Thus, we hypothesize that in the absence of pipetting, bacterial aggregate and heat treatment promotes formation of inter and intra bacterial bonding, leaving possibly the superior layer of this mass mostly exposed to AFM1. These results emphasize the implication of bacterial surface in AFM1 binding, this is in compliance with others who reported the implication of the bacterial cell wall polysaccharide and peptidoglycan as the two main elements in mutagens binding to LAB.29–32 Furthermore, the positive effect observed after heat treatment on AFM1 binding was in accordance with other previous studies.

  • Probiotics in Inflammatory Bowel Diseases and Cancer Prevention

    2016, Probiotics, Prebiotics, and Synbiotics: Bioactive Foods in Health Promotion
View all citing articles on Scopus
View full text