Elsevier

Clinica Chimica Acta

Volume 258, Issue 2, 17 February 1997, Pages 159-177
Clinica Chimica Acta

Monoclonal antibodies against human tetranectin, epitope characterization and use in immunohistochemistry

https://doi.org/10.1016/S0009-8981(96)06450-9Get rights and content

Abstract

Two IgG2a/κ- and three IgGl/κ monoclonal antibodies (MA's) were produced against human tetranectin (TN): this is the first report of stable hybridomas producing MA's against TN. All the MA's reacted with non-conformational epitopes located within amino acid residues 50–181 of the primary sequence. In relative epitope mapping with enzyme immunoassay and isotachophoresis the five MA's defined two independent epitope groups. One of them is suggested to be immunodominant in rabbits, since MA's inhibited binding of a polyclonal rabbit antibody. Several combinations of MA's were suitable for TN-ELISA and two MA's could be used for immunohistochemical detection of TN in both fresh frozen and paraffin-embedded tissues. The MA's will facilitate future studies on structure, function, clinical significance and immunolocalization of TN.

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    Dr. Inge Clemmensen died 27 August 1995.

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