Alimentary TractExperimental esophagitis induced by acid and pepsin in rabbits mimicking human reflux esophagitis☆,☆☆
Section snippets
Materials and methods
All animal studies were carried out in the Service of Biomedicine and Biomaterials of the University of Zaragoza, officially inscribed as a research establishment for the adequate husbandry and use of all research animals under the Good Laboratory Practices norms. New Zealand white rabbits weighing 2.5–3.5 kg each were studied. For surgical procedures, the animals were anesthetized with intramuscular ketamine hydrochloride (75 mg/kg; Parke–Davis, Madrid, Spain) and sodium thiobarbital (5 mm/kg;
Morbidity and mortality
Creation of a cutaneous cervicoesophageal fistula was explored initially but rejected because of extensive loss of food and salivary secretion. Placement of a metallic cannula in the same area was also explored but was not used because of biological rejection. One hundred forty-six rabbits were used with the plastic tubing system described in Materials and Methods; 117 (80%) of them were available for study analysis after completion of perfusions, and 29 (20%) were lost after the surgical
Discussion
To be useful, animal models of human diseases must reproduce, as closely as possible, pathological conditions observed in humans. The lack of appropriate animal models might be one of the reasons for the paucity of information on the mechanisms of damage and defense of the esophageal mucosa. In this study, 2 different and reproducible models of acid- and pepsin-induced chronic esophagitis are reported for the first time in rabbits. Rabbits had been previously used in models of acute esophagitis6
Acknowledgements
The authors thank Dr. E. Salvador for assistance with the perfusion regimen protocols and Sara Serrano and Professor Joaquín Soria for technical assistance with the mib1 studies.
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Supported by grants FIS 96/0872 and CYCYT PM-95-0204 from the Fondo de Investigaciones Sanitarias.
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Address requests for reprints to: Angel Lanas, M.D., Servicio de Aparato Digestivo, Hospital Clínico Universitario, 50009 Zaragoza, Spain. e-mail: [email protected]; fax: (34) 976-76-12-36.